Delmic Cryo-ET Symposium 2025: 7 experts share their latest advanced techniques and novel insights

Get to know the latest advances in cryo-electron tomography directly from the researchers. Curious how you can increase available milling sites and culture neurons more successfully on a TEM grid? Intrigued how to achieve magnitudes higher axial lamella targeting accuracy? Interested in what cryo-ET has revealed in multi-cellular organisms and tissue? Don't miss our exciting cryo-ET symposium! You'll get to dive deeper into the techniques and findings with our expert speakers.

In the 2025 symposium, we are focusing on two main topics:

1. The latest advanced cryo-ET techniques to increase sample yield
2. Revealing novel insights through in-tissue cryo-ET

You'll learn:

• How sandwich-like culturing kits help increase lamella generation sites on TEM grids
• How simultaneous FIB milling and fluorescence microscopy (FM) enables robust targeting of small, rare ROIs beyond the axial diffraction limit
• How FIB-angled FM imaging better targets elusive and rare events in multicellular samples
• How cryo-ET reveals synaptic, amyloid fibril and myelin protein structures within brain tissues
• How ice contamination minimisation helps increase context using serial lift-in (SOLIST)

Agenda:

6:00-6:10 pm (CEST) - Dr Katherine Lau, Delmic - Introduction to Session 1

6:10-6:25 pm (CEST) - Dr Shiwei Zhu, Case Western Reserve University, US

"SLICK: A Sandwich-Like Culturing Kit for in situ Cryo-ET Sample Preparation"

6:25-6:50 pm (CEST)- Dr Pete Dahlberg, Stanford University, US

"Registration-free approaches for the guidance of cryogenic focused ion beam milling with accuracy beyond the diffraction limit"

6:50-7:05 pm (CEST) - Cristina Capitanio and Dr Matthias Poge, Max Planck Institute for Biochemistry, DE

"'FIB-view': fluorescence imaging from the ion beam perspective guides milling of high-pressure-frozen samples"

7:05-7:20 pm (CEST) - All session 1 speakers - Panel Discussion

7:20-7:25 pm (CEST) - Dr Katherine Lau, Delmic - Introduction to Session 2

7:25-7:40 pm (CEST) - Dr Cathy Spangler, Vollum Institute, OHSU, US

"Cryo-ET investigation of native AMPA receptors at glutamatergic synapses within brain tissue"

7:40-7:55 pm (CEST) - Dr Yi Wei Chang, University of Pennsylvania, US

"Enabling cryo-ET imaging of human brain tissue by cryo-plasmaFIB sample preparation"

7:55-8:10 pm (CEST) - Mr Jean Daraspe, University of Lausanne, CH

"Cryo-FIB/SEM lamellae production as a service on an EM platform"

8:10-8:25 pm (CEST) - All session 2 speakers - Panel Discussion

8:25-8:30 pm (CEST) - Dr Katherine Lau, Delmic - Final remarks

For details on the abstracts, please visit our Delmic news page.

Sign up now to catch us live!