About this event
Developing transgenic animal models is essential for biomedical research — but confirming that a transgene is properly integrated and active can be challenging. Traditional methods often provide only partial sequence data and no insight into the surrounding regulatory landscape.
Fiber-seq changes that. This single-molecule, long-read multiomic method simultaneously maps chromatin accessibility, DNA methylation, and nucleotide sequence along individual fibers. Researchers can precisely verify integration sites and understand whether the transgene is accessible and ready for expression.
In this webinar, Zach Freeman, DVM, PhD, will show how his lab is using Fiber-seq to gain a complete picture of transgene integration and regulation. He’ll also discuss PacBio's PureTarget™ library preparation approach, which allows his lab to both increase throughput and maximize on-target sequencing to resolve gene-edited loci without amplification, preserving key data like endogenous methylation and Fiber-seq accessibility signal.
Additionally, Emily Madden, PhD (Product Manager, EpiCypher) will provide practical tips for getting started with Fiber-seq and highlight additional applications for this powerful technology.
Topics Covered
- Using Fiber-seq to verify transgene integration sites with single-molecule precision
- How Fiber-seq reveals local chromatin dynamics around inserted constructs
- Scaling throughput and reducing costs using custom PacBio PureTarget™ panels
- How Fiber-seq enables protein footprinting at near-nucleotide resolution
- How to get started with Fiber-seq!
Learn More About:
Hosted by
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Guest speaker
Zach Freeman, DVM, PhD Associate Professor @ Unit for Laboratory Animal Medicine at the University of MichiganG
Dr. Freeman is a veterinary scientist dedicated to advancing human medicine by improving the relevance of genetically-altered animal models. He completed his DVM at Colorado State University and his PhD in molecular biology and immunology from the Johns Hopkins University School of Medicine. Dr. Freeman is an Associate Professor in the Unit for Laboratory Animal Medicine at the University of Michigan and serves as the Director of the Transgenic Animal Model Core and Co Director of the NIH funded National Center of Rabbit Models for Translational Research. His research efforts have focused on improving the generation and characterization of genetically engineered animal models to ensure they closely model human disease. He has worked to develop new approaches leveraging long read sequencing and bioinformatics to improve the validation of genetically-altered models.
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Team member
Emily Madden Product Manager @ EpiCypher, Inc.T
Emily Madden is a Product Manager at EpiCypher, where she oversees the development and commercialization of innovative long-read sequencing technologies, including Fiber-seq, to advance epigenomic research.
EpiCypher
For Scientists, By Scientists.EpiCypher leads in chromatin mapping with its CUTANA® platform for ultra-sensitive ChIC, CUT&RUN, and CUT&Tag assays. We offer the largest collection of defined designer nucleosomes (dNucs), along with high-throughput assays and expert services to support researchers globally.
