About this event
In this webinar, Carina Emery, our partner Miltenyi Biotec's Product Manager for Sample Preparation, will discuss optimizing sample preparation for epigenomic assays such as CUTANA™ CUT&RUN/Tag. A strong sample preparation strategy tailored to your experimental needs lays the foundation for high-quality sequencing results. You will learn about the workflows from Miltenyi Biotec for reliable tissue dissociation, nuclei extraction, cell isolation, and sample cleanup designed to maximize sample quality for downstream epigenomic assays. You will also hear about the importance of isolating specific cell populations for bulk sequencing applications and share practical strategies to help you achieve more meaningful biological insights from your samples.
Then, EpiCypher’s Morgan Oatley will present on:
Tissue to Target: Optimizing Fresh Frozen Samples for CUTANA CUT&RUN Workflows
CUTANA™ CUT&RUN is fully compatible with tissue biopsies and primary cells, opening the door to chromatin profiling across sample types beyond cultured cell lines. In this webinar, we present a practical, generalizable nuclei dissociation protocol for use with CUTANA™ CUT&RUN and CUT&Tag workflows, covering manual and automated dissociation strategies across a range of tissue types. We'll walk through clean-up strategies for generating high-quality, intact nuclei – including approaches for removing unwanted debris – along with nuclei counting methods to ensure accurate inputs across your reactions.
Thank you to our guest speaker from:
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Carina Emery earned a BS from the University of Florida and an MS from Northwestern University where she studied the molecular basis of neurological disease. Carina has a passion for helping scientists utilize the latest technologies, and providing technical assistance to a wide variety of researchers has afforded her the firsthand knowledge that no matter how advanced your technology, you cannot compensate for a poor-quality sample. These days, as Lead Product Manager for Sample Preparation at Miltenyi Biotec, the dedicated biotech professional focuses on finding solutions to ensure researchers begin their experiments armed with the best possible starting materials.
Trained as a molecular biologist specialising in complex mammalian cell culture systems and transcriptomics. Now working to commercialise novel epigenomic technologies to advance drug development pipelines.
EpiCypher leads in chromatin mapping with its CUTANA® platform for ultra-sensitive ChIC, CUT&RUN, and CUT&Tag assays. We offer the largest collection of defined designer nucleosomes (dNucs), along with high-throughput assays and expert services to support researchers globally.