About this event
Mapping the genome-wide localization of transcription factors, chromatin modifying enzymes, histone post-translational modifications, and other chromatin-associated proteins is proving critical to biomedical research and therapeutic development. For the first time in decades, scientists now have multiple options for their chromatin mapping experiments. Though ChIP-seq has long been considered the gold standard epigenomic assay, advances in immunotethering technology have led to development of competing methods with dramatically improved sensitivity and throughput: CUT&RUN and CUT&Tag.
CUT&RUN and CUT&Tag generate high-quality epigenomic profiles with significantly reduced costs, hands-on time, and cell numbers compared to ChIP-seq. These advances are primarily accomplished by innovations in sample prep. Unlike ChIP, there is no cell lysis, cross-linking, chromatin fragmentation, or immunoprecipitation. With CUT&RUN and CUT&Tag, the entire assay takes place in intact cells bound to a solid support, allowing for streamlined sample processing and greatly reduced background. The resulting leap in sensitivity and throughput is expanding epigenomic applications in a way that ChIP never could: accessing precious primary cells and clinical samples, enabling automation and scale, and paving the way for next generation single-cell, spatial, and multiomic workflows.
EpiCypher scientists have set industry-wide standards for robust epigenomic mapping workflows. We have analyzed hundreds of sample types, tested thousands of antibodies, and performed more than 10,000 reactions. In this webinar, we will share our hard-won insights regarding ChIP-seq, CUT&RUN, and CUT&Tag, and explore how immunotethering assays are enabling novel epigenomic applications in cell & gene therapy, neuroscience, agriculture, and beyond. Tune in for answers to common questions:
Hosted by
Ellen is an expert in chromatin biology. She earned her PhD from the University of Pennsylvania and completed postdoctoral studies at the Jackson Laboratory for Genomic Medicine. At EpiCypher, Ellen leads scientific content and technical communications for products, services, and their applications.
Jen earned her MS at the University of Pittsburgh, working on the role of epigenetics in cell differentiation. After graduating, she joined EpiCypher, assisting researchers in performing CUT&RUN and CUT&Tag experiments and managing the Technical Sales and Support team operations.
EpiCypher is a world-leading provider of innovative research solutions to advance chromatin biology and improve human health. Take your project to the next level with EpiCypher's CUTANA™ CUT&RUN/CUT&Tag chromatin profiling assays and comprehensive class of nucleosome substrates and enzymes.
