Single Cell Sequencing: A New Dimension in Cancer Diagnosis and Treatment

A new multi-omics approach for single cell sequencing

Single cell analysis approaches are leading to new and impressive insights into complex biological systems, including the unique conditions of the tumour microenvironment. Heterogeneous sample types - such as tumour tissues - commonly harbour cell-specific genetic variants and gene expression profiles, both of which have been shown to be related to the mechanisms of disease development, progression, and responses to treatment.

However, most current high-throughput single cell sequencing methods detect only gene expression levels or epigenetics events such as chromatin conformation. To better decipher the connection between genotype and phenotype at single cell level, we need to develop and utilize new tools.

What this webinar will cover

This webinar will outline a new multi-omics approach for cancer research, the results from which have recently been published in Nature Communications (Wu et al., 2021 – full reference below). The case study focuses on single cell profiling of tumor heterogeneity and the microenvironment in advanced non-small cell lung cancer.

This webinar will also present two new technologies (used in the aforementioned case study) – FocuSCOPE and DynaSCOPE - that can detect which cells within a tumour carry a known mutation, and at the same time detect the gene expression in these particular cells. These unique solutions give a completely new dimension to single cell analysis.

What you will learn from this webinar

1. An overview and lessons learned from a recently published case study focusing on single cell profiling of tumour heterogeneity and the microenvironment.
2. An outline of new single cell multi-omics technologies used in the case study, which will enable you to:
3. Store fresh tissue/cells alive for 72 hours.
4. Effectively dissociate difficult tissue types (e.g., surgical biopsies, needle biopsies, lung, kidney, glioblastoma etc).
5. Detect mutations (SNP.Indel) or fusion genes simultaneously with single cell gene expression

Talks and Speakers

Talk 1: Comprehensive profiling of tumour heterogeneity and its microenvironment in advanced non-small cell lung cancer at single cell resolution.

Prof Margarete Odenthal, Head of the Laboratory of Translational Research at the Institute for Pathology, University of Cologne

----

Talk 2: FocuSCOPE: A multi-omics solution to simultaneously analyze genetic variants and transcriptomes in single cells.

Dr Nan Fang, CEO and Co-Founder, Singleron Biotechnologies

----

Talk 3: DynaSCOPE: A time-resolved single cell transcriptomic method to analyse transcription kinetics

Tore Bleckwehl, R&D Scientist, Singleron Biotechnologies

----

Q&A with all speakers

-----

Reference: A portion of the work presented in this webinar has just been published in the following paper:

Wu, F., Fan, J., He, Y. et al. Single-cell profiling of tumor heterogeneity and the microenvironment in advanced non-small cell lung cancer. Wu et al., 2021. Nat Commun 12, 2540 (2021) https://doi.org/10.1038/s41467-021-22801-0

----

This webinar has been produced with the kind support of Singleron Biotechnologies. You can find out more about the technologies outlined in this webinar by visiting the Singleron Biotechnologies website.