About this event
What if we could simultaneously retrieve spatial information about mRNA, neurotransmitters, post-translational and epigenetic modifications in 3D, with a highly multiplexed and accessible histology approach?
Over the past decade, the team of Dr Hei Ming LAI, at Chinese University of Hong Kong has been exploring novel 3D histology methods for clinical and research use. Published in Nature Methods, Nature Communications, ACS Applied Nano Materials, or Neuropathology and Applied Neurobiology, their work has also been awarded 3 Gold Medals in the International Exhibition of Inventions Geneva.
Just released on bioRxiv, their latest paper introduces INSIHGT (for IN SItu Host-Guest chemistry for Three-dimensional histology). This scalable and affordable approach can achieve uniform, deeply penetrating multiplexed immunohistochemistry in centimetre-scale samples.
Applied to whole mouse brains, INSIHGT was validated for 323 antibodies (including Immusmol antibodies against neurotransmitters) and 21 lectins, while preserving DNA integrity, thus allowing spatial transcriptomic approaches, including fluorescence in situ hybridization (FISH).
Excited to learn more about INSIHGT? Register for this quick 30-minute webinar!
Resources:
> Read the INSIHGT paper on bioRxiv
> To order INSIHGT staining solutions from Illumos (non-profit price)
> About Immusmol's primary antibodies for neurotransmitter imaging
Hosted by
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Guest speaker
Hei Ming Lai Clinical Assistant Professor, Department of Chemical Pathology @ The Chinese University of Hong KongG
Dr Hei Ming LAI is a Clinical Assistant Professor from the Department of Chemical Pathology at The Chinese University of Hong Kong. Over the past decade, he and his team have developed and perfected scalable and pragmatic 3D histology methods for clinical and research use. These works have been published in Nature Methods, Nature Communications, ACS Applied Nano Materials, and Neuropathology and Applied Neurobiology as the featured articles on the journal issue cover. The technologies were also recognized by the 3 Gold Medals in the International Exhibition of Inventions Geneva, an Honourable Mention in Nikon's Small World Photomicrography competition, and a Faculty Innovation Award, CUHK.
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Team member
Dominique BodetT
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Team member
Alban BessedeT
Immusmol
ImmuSmol develops small molecule antibodies for research, diagnostics, and therapy, unlocking new possibilities for detecting a wide range of small molecules.
