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In Tissue Cryo-ET: 5 experts share their latest advanced techniques and novel insights
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About this event
Get started with in-tissue cryo-ET and dive deeper into the molecular architectures of multicellular organisms, organoids, and tissue for novel insights with high physiological relevance.
Cryo-ET has enabled ultrastructural details such as protein-protein interaction and sub-organelle morphological details to be studied within cellular systems through focused ion beam (FIB) milling thin lamellae and imaging the lamellae in a cryo-transmission electron microscope (cryo-TEM). Single cells however are not the most physiologically relevant model for human biology. Thanks to recent advances in cryo-FIB technology and correlative workflows, biologists are able to dive deeper into larger, more physiologically relevant biological samples and unveil the sub-organelle ultrastructural details within. In this exciting mini-symposium, we invite expert cryo-electron tomography (cryo-ET) users and developers to tell us how they perform in-tissue cryo-ET to reveal novel important insights into key cellular processes and human diseases.
The mini-symposium offers you a chance to engage with leading experts in the field, where you can hear their research insights and ask questions about your own cryo-ET studies.
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Dominik studied microbiology at the Charles University in Prague, works in Electron microscopy core facility of the Institute of molecular genetics in Prague since 2017. He mainly focused on correlative and/or multimodal techniques and was a part of the collaboration between the Institute of molecular genetics and Tescan company on development of cryo FIB lift-out workflows.
Oda Helene Schiøtz is a PhD candidate at the Max-Planck Institute of Biochemistry in Munich. She started her PhD in 2020 in Professor Jürgen Plitzko’s lab for CryoEM Technology. There, her focus has been on methods development and their applications in cryo-ET. Specifically, her main area of research has been sample preparation and FIB-milling methods for large volume samples. She has applied these workflows to a variety of samples, including C. elegans and bobtail squid, and loves exploring the complex and rather confounding morphologies of the biology found within her lamellae. When a work day doesn’t quite go as well as planned, Oda takes her frustration to the quadball (previous quidditch) pitch, where she plays beater both on her local Munich team as well as the German National team.
Gong-Her Wu was born and raised in Taiwan. He completed his Ph.D. in the molecular and cellular biology department at National Tsing Hua University. He joined Dr. Wah Chiu’s lab as a Postdoctoral fellow in 2016. Throughout his career, Wu has significantly contributed to advancing cryo-EM techniques, particularly in developing novel imaging methodologies for visualizing high-resolution subcellular structures. His pioneering work includes establishing the workflow for integrating cryo-confocal, cryo-plasma-focused ion beam (cryo-pFIB) milling with scanning electron microscopy (SEM) and cryo-ET, enabling detailed three-dimensional visualization of cellular components without the need for chemical fixation or staining. In addition, he applied these novel multiple cryo-microscopy imaging technologies to investigate Huntington’s disease.
Philipp S Erdmann is a chemical biologist and cryo-electron microscopist. After receiving his PhD from the LMU in Munich, he joined Alice Y Ting at MIT to work on the proteome and ultrastructure of excitatory and inhibitory synapses using APEX2 labeling. After returning to Munich, Philipp joined the team of Wolfgang Baumeister and Jürgen Plitzko to develop methods enabling correlative high-resolution cryo-electron tomography inside cells. Since 2021, Philipp has been a group leader at Human Technopole Milan, Italy, working on resolving the structures of liquid-liquid phase-separated compartments involved in human diseases.
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