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Delmic Cryo-ET Journal Club Series - Nature protocol: Cryo-ET sample preparation from whole cells

About this event

A protocol named “Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography” has been published recently on Nature. Several concepts and optimizations for the technique have already been published before, but a detailed protocol such as the one presented here will certainly help a lot of researchers getting to grips with the complex workflow. Join our Cryo-ET Journal Club to discuss this technique with your peers!

A journal club on a methods article might seem a bit strange, but to have a full protocol published for such a complex method is quite the milestone for the cryo-ET technique. In this Journal club, our hosts Dr. Caspar Jonker and Marit Smeets will discuss the workflow described by Wagner et al. and compare It to other workflows that have been described over time. We will also dive into the equipment and materials described in the article and discuss which technical advances might make this protocol a little easier in the future.

We invite you to read the preprints before the journal club to prepare for an interesting discussion:

Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography

If you would like to stay updated about the upcoming episodes, you can subscribe to the whole series here!

Hosted by

  • Guest speaker
    G
    Marit Smeets

Delmic

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