Using Single Cell Multi-Omics to Analyse the Immune System

Improving our understanding of how the immune system functions in health and disease will lead to the development of more effective therapies for a wide range of diseases.

Single cell sequencing is a valuable approach for studying the immune system and its crosstalk with various tissues, providing information on gene expression within thousands of cells in a single experiment. Now that a new era of single cell multi-omics has emerged, new layers of information can be added to transcriptomics data, allowing us to study biological processes with unprecedent detail and throughput.

But – how can you best use these new approaches to take your own research forwards? That’s where this webinar will help you...

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What will be covered?

The first talk – delivered by Dr. Anke Faehnrich of the University of Luebeck, Germany – will present recent findings on the crosstalk between immune and epithelial cells in post-COVID patients, as analysed with single cell RNA sequencing of nasal biopsies.

What you will learn from this talk:

An outline of applying single cell RNA-seq to study nasal biopsies, including:

• Transcriptome profiles of the identified cell types.
• Identification of key molecules and biological pathways linked to post-COVID syndrome.
• Immune/ epithelial crosstalk and its potential effect on mucosal barrier function.

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The second talk – delivered by Prof. Jie P. Li, of the Nanjing University, China – will introduce a novel multi-omic approach for the quantification of cell surface glycosylation, together with gene profiling at single cell level.

What you will learn from this talk:

• The working principles of a novel chemoenzymatic method for LacNac detection at a single cell level.
• How cell-surface glycosylation levels vary in activated and quiescent CD8+ T cells.
• Further applications of single cell multi-omic approaches.

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Talks and Speakers

Talk 1: Single cell RNA sequencing from nasal biopsies reveals immune-/epithelial cell cross-talk in symptomatic post-COVID patients

Dr. Anke Faehnrich, Head of the Single Cell Core Facility and Single Cell Sequencing Section, Institute of Experimental Dermatology, University of Luebeck, Germany

Talk 2: Chemoenzymatic measurement of cell-surface LacNAc in single cell multi-omics reveals LacNAc as a cell-surface indicator of glycolytic activity of CD8+ T cells

Prof. Jie P. Li, Professor of Chemical Immunology, Nanjing University, China

Talks are followed by audience Q&A with both speakers.

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This webinar has been produced with the kind support of Singleron Biotechnologies. You can find out more about the technologies outlined in this webinar by visiting the Singleron Biotechnologies website.