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About this event
Join our webinar on advancing your research with integrated fluorescence microscopy and focused ion beam milling (cryo-FM-FIB)! Explore the potential of precision targeting to revolutionize your work in fluorescence-guided cryoFIB, cryoET, or CLEM. You’ll get to learn directly from experts as they unveil insights from their experiences, including a featured presentation showcasing the groundbreaking work achieved with our fluorescent light microscopy: an integrated FIB/SEM. Sign up now!
Key takeaways for viewers:
Abstracts:
Speaker: Phyllis Wang
Position: PhD Student at Stanford Linear Accelerator Center (SLAC)
Title: Tri-coincident FM-FIB-SEM platform for sub-diffraction limited targeted milling
Abstract: Integrated cryo-fluorescence microscopy and focused ion beam milling (cryo-FM-FIB) is a state-of-the-art technique for capturing fluorescently labeled targets in biological specimens and retaining them in thin lamellae for subsequent cryogenic electron tomography. Though previous approaches have applied to visualize fluorescent targets in vitrified cells, a lack of precision in target localization precludes its broader application to routinely capture small and rare structures of interest in situ. Our current work focuses on utilizing a customized integrated tri-coincident imaging system that allows for simultaneous fluorescence imaging and milling to visualize challenging sub-diffraction limited fluorescent biological targets with high fidelity. We have successfully localized the microtubule organizing center (MTOC) in vitrified mammalian cells as a crucial organelle responsible for cell division and cell differentiation. Tomographic reconstructions also revealed organization of microtubules at the MTOC and interactions with other organelles.
Speaker: Wessel Teunisse
Position: Application Specialist Cryo at Delmic
Title: Tri-coincident beam imaging vs. Delmic’s best in class integrated light microscope METEOR.
Abstract:
Tri-coincident beam (FIB/SEM/FLM) imaging is an exciting imaging approach for correlative cryo-FIB milling. How does this technique compare to the currently commercially available high-quality integrated light microscope METEOR? In this talk the advantages and disadvantages of approaches will be highlighted. We will conclude with a discussion on whether your research requires a tri-coincident beam system or that a non-coincident system might offer some unexpected benefits.
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