3D Cryo-CLEM | New Insights Using Accurate Cryo-Fluorescence Correlation Tools

How can you increase your cryo-fluorescence resolution using your current microscope? How can you navigate in a 3D tissue sample for 3D FIB/SEM volume imaging? Learn how to leverage the right tools to streamline your cryo-EM and cryo-CLEM workflows without needing to buy expensive equipment. Sign up for our upcoming webinar, and learn from the experts!

What to expect:

• Discover how to increase your cryo-fluorescence resolution
• Learn how to easily navigate 3D tissue samples
• Gain insights into the latest 3D cryo-CLEM research 
• Ask your questions in the interactive discussions 

More information:

Cryo-correlative light and electron microscopy (cryo-CLEM) is a powerful technique to navigate biological samples that are imaged at high resolution. Most cryo-CLEM workflows use cryo-light microscopy (cryo-LM) and cryo-electron tomography (cryo-ET) to visualize single cells in two dimensions (2D), lacking the contextual information that comes from 3D samples. However, applying cryo-CLEM to 3D samples like tissues or organoids faces new challenges such as a resolution gap between cryo-LM and cryo-ET. Tools have already been developed to overcome this challenge, but they are not yet widely used. 

In this webinar, Delmic’s application specialist Marit de Beer will talk about navigating samples for 3D volume cryoFIB/SEM imaging using cryo-air scan confocal microscopy and an imprint of a finderTOP pattern on the sample surface during high-pressure freezing. In addition, Peter Kirchweger, a postdoctoral fellow from the Weizmann Institute of Science, will talk about using cryo-Super Resolution Radial Fluctuation (cryo-SRRF) microscopy to increase the fluorescence resolution and successfully image fibroblast cells in high resolution using cryo-CLEM.